Master of Science in
Graduation Date: May 2004
Thesis Topic: Detection, Predictors, Immune Mechanisms and
Prevalence of Porcine
Reproductive and Respiratory Syndrome (PRRSV) Persistence in
Bachelor of Science in Arts and Science
Major: Biology and Microbiology
(8/04 – present)
taught: General Biology I and II, Medical Microbiology,
Genetics, Immunology, Human Biology, Aspects of Human
Disease, Senior Research and Biology Seminar
and coordinate lab experiments
Co-advise Biology club
Graduate Teaching Assistant
Biology and Microbiology Department,
General Biology I and II and General Microbiology labs
Coordinate lab experiments
and administer weekly lab quizzes and semester tests
size ranged from 15 to 30 students
Developed and validated a real-time PCR assay for the
detection of PRRSV in serum and semen.
Performed conventional PCR, RNA extractions, virus titration
and isolation, serology testing, reagent production, and
monoclonal antibody production.
Conducted small and large animal challenge studies, and
Benchmark Biolabs, Inc., Lincoln, NE.
(9/00 – 8/01)
microbial and biochemical tasks associated with data
tasks associated with automated and manual assays.
Research Technician II
Intervet Animal Health,
(9/99 – 9/00)
Performed cell culture, virus titration, serology testing,
embroynated egg titrations, inoculations and harvesting.
Performance of small and large animal immunogenicity
studies, including vaccination, challenge, monitoring of
clinical signs, collection of samples.
Lu, W., M. Eichmeyer, J.
Schroeder, R. Atchison,
Preliminary Characterization of
a H3 Subtype Isolation of Swine Influenza Virus.
of Swine Practitioners. 119-122.
Wasilk, A., J.D.
Callahan, J. Christopher-Hennings
et al. 2004.
Detection of U.S. and LV/European-like porcine
reproductive and respiratory syndrome virus and quantitation
in boar semen and serum by real-time PCR.
Journal of Clinical Microbiology.
Published Abstracts and
Wasilk, A., J.
Christopher-Hennings, J. Callahan, T. Gay, Y. Fang, M.
Dammon, M. Torremorell, E.A.
Nelson, W.M. Nelson.
2003. Validation of a real-time
quantitative PCR assay to detect US and Lelystad/European-like
PRRSV in boar semen and serum.
84th Conf. of
Research Workers in Animal Disease.
Callahan, J.D., J.
Christopher-Hennings, T.A. Gay, M.E. Reos, Y. Fang,
A. Wasilk, J. Galeota, F.A. Osorio, M. Torremorell, W.M. Nelson and
E.A. Nelson. 2003. Development, validation and
commercialization of a real-time PCR assay for the detection
of Lelystad/European-like and U.S. PRRSV.
107th Annual Meeting
of the American Association of Vet. Lab. Diagnostitions, p.
Page, B., Y. Fang,
S. Ropp, N. Ferrin,
A. Wasilk, P. Steen, J.Christopher-Hennings and E.A.
Identification of European-like
PRRSV in the United States.
Proceedings of the
of Science. Vol. 82.
Wasilk, A., D.L.
Carlson, Y. Fang, S.A.E. Marras, E.A. Nelson and J.
2002. Detection, differentiation
and quantitation of PRRSV strains by real-time PCR with
molecular beacons. 83rd Conf. of Research Workers
in Animal Disease.
St. Louis, MO.
Fraser, T., J.
Christopher-Hennings, K. Rossow, E. Thacker, P. Ahrens, R.
Chapin, A. Wasilk
and E.A. Nelson.
hyopneumoniae by nested PCR:
A comparison of specimen type
and extraction methods. American Association of Veterinary
American Association for the Advancement
Faculty Welfare Committee
Member (2004-06) Chairperson
Chemistry and Physic’s Faculty
Search Committees (2006)
Women in Science committee
member (2006- present)
Ad Hoc: Graduation Requirements/Grades Committee (2006-2007)
College Development Committee
member (2007- present)